FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22015472
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4478222.0341240015203854No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGTCA69500.017.8503592
TCTAGAC87000.013.4998357
TGCCGTG50950.013.233641145
CGAGGTC91600.012.8974141
ATTCTAG108200.012.596668
ATGCCGG55950.012.051009145
CATTCTA117300.011.8050557
CTAGACT99250.011.6873028
TTCTAGA128900.010.5735999
TAGACTT114200.010.4110779
GAGCACC72150.010.249541145
GGATAGT30500.010.0422861
AGACTTC114550.09.7494833
CGGGCAT23800.09.1923681
TTCGTAC109900.09.1721923
CGTACAA120150.09.0511165
CGTCTTG61650.08.937604145
CGTCCCA73450.08.784628
GTCCCAT76100.08.7643779
CCCGTAT26100.08.6617231
Produced by FastQC (version 0.11.5)