FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n01_bulk_9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n01_bulk_9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21944484
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTCACACCGA807940.3681745262271831No Hit
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT680520.3101098207640699No Hit
ATAGATATTAATTTTATTCTTAAAATAAAAAATATTTTTCATAAACAATC633460.28866479612826623No Hit
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT607400.27678937449611485No Hit
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG466110.21240417409677986No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT447450.20390089828496308No Hit
GTCTTTTCGTCCCATAAATTTATTTAAGTTTTCTTACTTAAAAAATAAAT438250.19970850077860114No Hit
TTTTATTATAGATATTAATTTTATTCTTAAAATAAAAAATATTTTTCATA403430.18384118760778334No Hit
ATTTTATTCTTAAAATAAAAAATATTTTTCATAAACAATCCTTTCGTACA391970.17861891854007594No Hit
TATAGATATTAATTTTATTCTTAAAATAAAAAATATTTTTCATAAACAAT379500.17293639713743097No Hit
CTGGCTCACACCGATCTAAACTCAAATCATGTAAGATTTTAATAATCGAA369700.16847058240239324No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC365430.16652476312498393No Hit
TTTTATTCTTAAAATAAAAAATATTTTTCATAAACAATCCTTTCGTACAA343910.15671819852314595No Hit
CCCAATTATATATATACTACAAAATTAAATTATGCTGTTATCCCTAAGGT328800.14983264131432755No Hit
CATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGTTATCCC294330.13412482152690397No Hit
GTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGTTATC243270.11085701536659508No Hit
TAGATATTAATTTTATTCTTAAAATAAAAAATATTTTTCATAAACAATCC240080.1094033471008022No Hit
CTCACACCGATCTAAACTCAAATCATGTAAGATTTTAATAATCGAACAGA236850.10793145101976424No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGATG253450.022.454802145
TAGATAT460550.021.9609972
GGTCATA496000.021.868624
GATATTA505700.020.8613054
GAGGTCA496550.020.1059952
AGGTCAT501750.019.9845053
ATGCCGG126650.019.806274145
TAATCCC553250.019.6047299
GGATAGT105500.018.005061
GTCATAA609300.017.9212885
ATTATAG410950.017.5024195
GTCCCAT415300.017.4403119
CGTCCCA420150.017.1358438
CGAGGTC570900.016.8395021
TCGTCCC440800.016.596717
AGATATT621350.016.5578783
ATAATCC689100.015.9506198
CATAATC706500.015.4761047
CGTACAA753200.015.1712485
TTCGTAC737950.015.0616943
Produced by FastQC (version 0.11.5)