FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n01_bulk_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n01_bulk_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23182739
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT432270.18646200520136988No Hit
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG414170.1786544721915732No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC402960.17381897799047819No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT379060.16350958357422735No Hit
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTTACACCGA365620.15771216679789216No Hit
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT333830.14399937815803387No Hit
GTCTTTTCGTCCCATAAATTTATTTAAGTTTTCTTACTTAAAAAATAAAT314050.13546716805119532No Hit
TTTTATTCTTAAAATAAAAAATATTTTTTATAAACAATCCTTTCGTACAA256110.11047443531154795No Hit
ATTTTATTCTTAAAATAAAAAATATTTTTTATAAACAATCCTTTCGTACA251570.1085160817278752No Hit
ATAGATATTAATTTTATTCTTAAAATAAAAAATATTTTTTATAAACAATC244830.1056087462314095No Hit
CCCAATTATATATATACTACAAAATTAAATTATGCTGTTATCCCTAAGGT237640.10250730079823614No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA311100.023.5849914
GAGGTCA321550.021.4649812
AGGTCAT326300.021.3526693
TAATCCC379550.019.5031789
TTGAATG327050.017.689741145
GTCATAA427200.017.4639745
CGAGGTC398650.016.9497181
TCTAGAC440300.014.7377277
ATAATCC515050.014.6257958
CATAATC522450.014.3632187
GTCCCAT328900.014.3284149
CGTCCCA337550.013.9399088
CCAGTCC136500.013.278228145
ATTCTAG529350.013.1212728
CTAGACT499250.013.0119768
CATTCTA543600.012.9907654
TCGTCCC375600.012.8752727
TCATAAT622600.012.2624776
TAGATAT348500.012.0245042
AAGAGCC174650.011.9551935145
Produced by FastQC (version 0.11.5)