FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n01_bulk_7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n01_bulk_7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25407332
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT441580.1738002242817152No Hit
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG394040.15508909003117682No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT383120.15079111809142337No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC370360.14576894575156493No Hit
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTTACACCGA365760.14395844475130248No Hit
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT329310.1296121922600925No Hit
GTCTTTTCGTCCCATAAATTTATTTAAGTTTTCTTACTTAAAAAATAAAT318730.12544803995948886No Hit
ATAGATATTAATTTTATTCTTAAAATAAAAAATATTTTTTATAAACAATC271180.10673296983721077No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA329600.023.7795264
GAGGTCA339400.021.7461642
AGGTCAT346200.021.2146953
TAATCCC399400.019.4789
GATCATG373100.018.67377145
GTCATAA448500.017.815065
CGAGGTC439050.016.6121731
CCAGTCC172700.014.73495145
ATAATCC549000.014.6855248
CGTATGG162000.014.634122145
GTCCCAT349550.014.6021269
CATAATC551300.014.4799357
CGTCCCA355600.014.1907218
TCTAGAC447650.013.3947367
CTAGACT506650.012.5497191
TCGTCCC416100.012.5284817
TCATAAT658650.012.4281756
TTAAACC192750.011.9242787
CATTCTA563950.011.738024
GATATTA422600.011.7352214
Produced by FastQC (version 0.11.5)