FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n01_bulk_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n01_bulk_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23264545
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG414870.1783271497465349No Hit
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT396270.1703321513487584No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT363370.15619046063441172No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC336870.14479973711069785No Hit
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTTACACCGA318910.1370798354319846No Hit
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT317030.1362717388197362No Hit
GTCTTTTCGTCCCATAAATTTATTTAAGTTTTCTTACTTAAAAAATAAAT307910.1323516105730845No Hit
ATAGATATTAATTTTATTCTTAAAATAAAAAATATTTTTTATAAACAATC234220.1006768024046892No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA284800.024.4144654
ATGCCGG184100.022.40731145
GAGGTCA295450.022.1344972
AGGTCAT301450.021.6462543
TAATCCC351150.019.5943539
GTCATAA396000.018.0166155
TGCCGTG187700.017.61294145
CGTATGG112100.017.591164145
CGAGGTC388250.016.5075041
GTCCCAT317200.015.08579359
ATAATCC487100.014.8104728
CATAATC495100.014.5274127
CGTCCCA327600.014.4964398
ATTCTAG496650.013.5475728
AAGAGCC181450.013.385022145
CATTCTA523350.013.0643857
TCGTCCC376600.013.0147537
CGTACAA456800.012.7139265
CGTCTTG241350.012.706452145
TCATAAT583750.012.53249556
Produced by FastQC (version 0.11.5)