FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n01_bulk_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n01_bulk_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23442041
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTCACACCGA443500.18919001122811788No Hit
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT409470.1746733571535004No Hit
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG376810.16074112318121106No Hit
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT369430.1575929331409326No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT334170.14255158072626867No Hit
ATAGATATTAATTTTATTCTTAAAATAAAAAATATTTTTCATAAACAATC327830.13984703806294No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC287800.12277087989053513No Hit
GTCTTTTCGTCCCATAAATTTATTTAAGTTTTCTTACTTAAAAAATAAAT284220.12124370911218865No Hit
CTGGCTCACACCGATCTAAACTCAAATCATGTAAGATTTTAATAATCGAA260240.11101422440136506No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA324250.020.214144
CGTATGG171600.018.16694145
AGGTCAT339350.018.1176873
GAGGTCA339050.018.090642
TAATCCC381600.017.1566779
ATGCCGG245600.016.235476145
GTCATAA432750.015.447755
TAGATAT378800.015.0821612
GTCCCAT309300.014.5802269
CGTCCCA312450.014.2941598
GATATTA434650.014.27914
CGAGGTC426800.013.9973391
ACAGTGC262650.013.911815145
CGTACAA517400.013.5369895
TTCGTAC515100.013.0198183
ATAATCC517150.012.9542998
CATAATC528550.012.7162917
TCGTCCC371100.012.4651227
ATTCTAG475650.012.270638
ATTATAG333400.012.2437435
Produced by FastQC (version 0.11.5)