FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n01_bulk_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n01_bulk_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18891807
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG326690.17292681425339565No Hit
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT300540.15908483502928014No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT270750.14331609464356693No Hit
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT251130.13293064025055942No Hit
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTTACACCGA237130.1255200203982605No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC221490.11724129936326365No Hit
GTCTTTTCGTCCCATAAATTTATTTAAGTTTTCTTACTTAAAAAATAAAT219890.11639437138014379No Hit
ATAGATATTAATTTTATTCTTAAAATAAAAAATATTTTTTATAAACAATC199010.10534196120042937No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGCC122750.025.337898145
GGTCATA222200.023.3303344
AGGCATG328000.021.904564145
GAGGTCA232050.021.1207392
AGGTCAT236400.020.6098043
TAATCCC267100.019.4895349
CGTATGG127900.018.365774145
GTCATAA308500.017.3209785
CGAGGTC296850.015.9484251
ATGCCGG172700.015.826461145
GTCCCAT247800.014.3658439
ATAATCC374950.014.0770358
CGTCCCA254400.013.9932178
ATTCTAG400650.013.5721718
CATAATC391200.013.3441667
TGCCGTG172650.013.269523145
CATTCTA418450.013.133597
AAGAGCC157550.012.654619145
TCGTCCC296350.012.3061117
CTAGACT374000.012.1932861
Produced by FastQC (version 0.11.5)