FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n01_bulk_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n01_bulk_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24125729
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG326920.13550678613690803No Hit
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT294410.12203154565816436No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC274590.11381624986337201No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT266000.11025573569196603No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA230700.022.3137654
ATGCCGG175550.020.938251145
GAGGTCA239800.020.2871512
AGGTCAT247100.019.6000353
TAATCCC293100.017.4638589
CACGATG427800.015.997957145
TGCCGTG183200.015.90867145
ATCACGC368450.015.584036145
GTCATAA342650.015.5314155
CGTATGG96250.014.085561145
CGAGGTC374150.012.9442421
TCTAGAC353900.012.8659577
AAGAGCC193050.012.806129145
ATAATCC421950.012.6465148
ATTCTAG425650.012.6387838
CGTCTTG244300.012.285992145
CGTCCCA291050.012.1813468
CATAATC436850.012.0494527
CATTCTA454800.011.8448987
GTCCCAT302000.011.5475399
Produced by FastQC (version 0.11.5)