FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n01_bulk_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n01_bulk_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21071022
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC365290.17336131109350084No Hit
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG329850.156542003515539No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA163200.020.924764
AGGTCAT181100.017.5352233
GAGGTCA184250.017.4318962
TCTAGAC328900.016.6213467
TAATCCC216150.016.3014939
ATTCTAG367950.015.46826655
CTAGACT354400.015.3638048
CATTCTA417100.014.1843594
ACCAATG154550.013.510066145
GTCATAA262250.013.4916525
TAGACTT412300.013.4698199
AAGAGCC159050.013.1734085145
TTCTAGA435950.012.88919456
TCACTGC192850.012.142746145
CCTTCAT470350.011.4685213
CGAGGTC273550.011.1315291
ATAATCC335600.011.1043638
ATGCCGG127900.010.996771145
GAGCACC185050.010.734838145
TCATTCT576800.010.6089613
Produced by FastQC (version 0.11.5)