FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n01_bulk_24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n01_bulk_24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22380624
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG204750.024.04241145
AGCCAGG94850.023.3910244
CGTATGG142100.022.90785145
AGGCGTG106300.021.6898738
TGCCGTG204950.021.47202145
CTTTATG277800.020.356096145
GCCAGGC115000.019.7971085
CTCAGCC127300.019.6483761
GGCGTGG123700.018.8143739
CAGGCGT125150.018.4231687
GGTCATA164450.017.062484
CCAGGCG145500.015.9960586
GAGGTCA173800.015.142642
CAGCCAG162550.014.3175783
TTATCTG228550.013.95737145
TCAGCCA182900.012.9225622
AGGTCAT214400.012.782583
CTAGACT228200.012.6445511
GTCCCTA106100.012.3687658
CCCTAGG46350.012.32562120-24
Produced by FastQC (version 0.11.5)