FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n01_bulk_23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n01_bulk_23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23045057
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTCACACCGA255660.11093919186227225No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA215000.016.2546674
ATGCCGG142100.016.020214145
GAGGTCA223250.014.7439582
TGCCGTG160500.014.635337145
AGGTCAT232450.013.9736073
TAATCCC272250.012.7828259
TCTAGAC258950.012.4878717
TTAAACC176700.012.3098917
CGTATGG80950.012.180211145
CTAGACT290500.012.1541611
TAAACCT173950.011.6289588
GTCATAA315150.011.5033435
AAGAGCC194700.011.208134145
CCAGTCC137000.010.742571145
GTATGCC1020550.010.734033145
CATTCTA351450.010.6865054
CTCATTT288700.010.5473811
ATTCTAG345800.010.4834948
CGTCTTG235750.010.363607145
GCCGTCT752500.010.212501145
Produced by FastQC (version 0.11.5)