FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n01_bulk_22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n01_bulk_22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22592039
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG229110.10141182918460791No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATATG216950.023.459177145
ATGCCGG155750.022.622654145
GGTCATA192950.020.6668554
GAGGTCA200950.018.4004752
AGGTCAT211850.017.488163
CGTATGG100450.017.24977145
TGCCGTG189450.016.799843145
TAATCCC246400.016.2422169
GTCATAA297450.013.8450245
CGAGGTC283000.013.1679371
GAGTGGT80850.012.195349145
CTAGACT282100.011.8735331
ATTCTAG307550.011.7163758
CGTCTTG230900.011.146531145
GTCCCAT215950.011.0456069
ATAATCC381300.011.0284348
AAGAGCC175600.011.023565145
TCTAGAC247600.010.8931057
CATTCTA336200.010.8259347
CATAATC390750.010.7618867
Produced by FastQC (version 0.11.5)