FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n01_bulk_21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n01_bulk_21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22831298
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGG138750.020.2214145
ATGCCGG188700.020.093842145
GGTCATA210750.019.8500424
GAGGTCA219200.017.9596632
CGTAATG316600.017.678265145
AGGTCAT228450.017.2326983
TAATCCC261300.015.8709589
TGCCGTG208150.015.464638145
GTCATAA308700.014.06835655
TCTAGAC247400.013.246227
CGAGGTC306000.012.8651681
CCAGTCC162400.012.857145
AAGAGCC168050.012.640444145
ATTCTAG305700.011.6212785
CTAGACT280900.011.485688
GTCCCAT213450.011.3108339
ATAATCC389050.011.2931648
CATTCTA338350.011.0141294
CATAATC400500.010.9704217
CGTCTTG244500.010.556121145
Produced by FastQC (version 0.11.5)