FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n01_bulk_20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n01_bulk_20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22442345
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTCACACCGA298060.1328114330298371No Hit
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT259210.11550040782280105No Hit
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG241810.10774720734397407No Hit
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT241760.10772492803225331No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT237050.10562621686815706No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA224900.021.4061644
CGGAATG309250.021.169527145
GAGGTCA233150.019.155562
AGGTCAT243100.018.4909083
TAATCCC280350.017.2233059
GTCATAA328300.014.9955375
CGAGGTC314350.014.0918061
GTCCCAT238400.012.3774449
CTAGACT311550.012.24043751
ATAATCC415000.012.1245558
TCTAGAC279100.011.9496817
CATAATC423550.011.948387
CGTCCCA252550.011.8565178
ATTCTAG349100.011.3603558
TAGATAT321850.011.1056252
GGTTGGG103400.011.078212145
TTAAACC167350.010.9177497
GGGGGTT130000.010.874878145
CATTCTA368950.010.8671567
CCAGTCC127150.010.719502145
Produced by FastQC (version 0.11.5)