FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n01_bulk_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n01_bulk_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21179567
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG383110.1808866064164579No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC380020.17942765307713798No Hit
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT240770.11368032217089236No Hit
GTCTTTTCGTCCCATAAATTTATTTAAGTTTTCTTACTTAAAAAATAAAT220150.10394452351174129No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA186000.023.3883634
AGGTCAT204100.019.5731563
GAGGTCA214600.019.3246862
TAATCCC239550.018.0384529
TCTAGAC367600.015.6211217
GTCATAA291900.015.3503385
ATTCTAG409350.014.877918
CTAGACT400050.014.3719718
AAGAGCC150850.013.937586145
CATTCTA450000.013.6951854
CGAGGTC312850.013.0469861
ATAATCC359700.012.7994328
TAGACTT459400.012.7043989
TTCTAGA488900.012.5013459
GTCCCAT278450.012.0814759
CTCCCAA212650.011.830471
CCTTCAT530050.011.7497493
CGTCCCA288200.011.6729728
CATAATC378050.011.5645997
CCCAATA199950.011.240683
Produced by FastQC (version 0.11.5)