FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n01_bulk_19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n01_bulk_19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23024100
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTCACACCGA239220.1038998267033239No Hit
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG237120.10298773893442088No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTATG164200.030.333244145
CGTATGG138150.020.25684145
GGTCATA213950.019.7905774
ATGCCGG210450.019.46412145
TGCCGTG209250.017.947317145
AGGTCAT230700.017.0018543
GAGGTCA228950.016.878292
GGCCTTT132300.015.837013145
TAATCCC269400.015.4208189
GTCATAA320400.013.5096025
GTGGCCG199250.013.462901145
TCTAGAC276700.012.7084167
CGAGGTC309400.012.3958231
GTCCCAT231400.011.46748359
CTTATCG453700.011.329553145
TTATCTG239700.011.221247145
AAGAGCC180500.011.2063145
CGTCCCA238050.011.1167528
CTAGACT311250.011.0413498
ATTCTAG348400.010.9879465
Produced by FastQC (version 0.11.5)