FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n01_bulk_18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n01_bulk_18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22393584
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT316940.14153161012547164No Hit
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG263320.11758725177711615No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT256890.11471589362381654No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC240730.10749954093994066No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA224300.024.7612134
GAGGTCA234350.022.6152612
AGGTCAT239600.021.9085453
TAATCCC279100.019.6388479
GTCATAA319400.017.3433445
AACGATG373800.017.145323145
ATGCCGG140100.016.19716145
CGAGGTC340550.015.32819751
TGCCGTG163850.014.291861145
CATAATC411800.013.8918537
ATAATCC412550.013.6906678
GTCCCAT260550.013.0507599
TCTAGAC318250.013.00880057
CGTCCCA275500.012.6586458
GAAACGC410600.012.324496145
GGATAGT92400.012.0833741
ATTCTAG385850.011.6315048
CTAGACT361200.011.5012861
TCATAAT505100.011.4838366
CATTCTA417700.011.1093324
Produced by FastQC (version 0.11.5)