FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n01_bulk_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n01_bulk_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23203773
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG235340.10142316079372092No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG181950.023.190289145
GGTCATA207350.019.3364984
GTCCGCC279050.018.602282145
CGTATGG100900.017.460245145
GAGGTCA215450.017.3302332
AGGTCAT224350.016.642993
TGCCGTG187000.015.856851145
TAATCCC263500.015.2706969
CCGCACC273900.014.796364145
GCACATG208650.014.663218145
GTCATAA313950.013.4868645
CCAGTCC153900.012.342351145
CTAGACT285250.012.2507111
CGAGGTC310200.012.1301311
CGTCTTG236250.012.060239145
AAGAGCC200700.011.956824145
ATTCTAG317900.011.6313688
TCTAGAC255400.011.5823077
CACATCG324350.011.578467145
ATAATCC385300.011.0832578
Produced by FastQC (version 0.11.5)