FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n01_bulk_16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n01_bulk_16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22003905
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT238810.10853073579439651No Hit
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG235350.10695828763121817No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA181950.021.35874
CCGCATG159850.020.908539145
GAGGTCA197300.018.5569932
CGTATGG155000.018.101505145
AGGTCAT201600.018.0178643
GTCCGCC340650.016.643082145
TAATCCC242150.016.3778179
ATGCCGG223100.016.248222145
CCAGTCC240150.014.943696145
GTCATAA278100.014.23494155
GCATCTG239750.012.912332145
CACCCGG94850.012.764815145
CGAGGTC284450.012.4635651
TGCCGTG200000.012.433676145
TCTAGAC270150.012.1578647
ATTCTAG323150.012.0707788
AAGAGCC175650.011.639554145
CATTCTA351250.011.45620257
CTAGACT304700.011.3735171
AACTCCC118900.011.158469145
Produced by FastQC (version 0.11.5)