FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n01_bulk_15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n01_bulk_15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23101267
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG296040.12814881538748502No Hit
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT258130.11173846005935519No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC253750.10984246015597325No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT250970.10863906295702311No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA202550.022.4077854
CAGAATG271250.020.126139145
GAGGTCA213050.019.9077742
AGGTCAT217550.019.6628283
TAATCCC259500.017.6573039
GTCATAA309500.015.0629485
CGAGGTC313100.013.4071181
TCTAGAC311000.013.0319717
ATTCTAG376900.012.9077178
AAGAGCC188100.012.75776145
ATAATCC383250.012.542518
CATTCTA406750.012.0853957
CATAATC399800.012.0053257
CTAGACT340550.011.8158558
GAATCTG373300.010.85648145
TTCTAGA460250.010.7274859
GAATCGG566250.010.703684145
CGTCCCA251300.010.588318
CCTTCAT484550.010.5039253
GACTTCA409250.010.3993514
Produced by FastQC (version 0.11.5)