FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n01_bulk_14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n01_bulk_14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20667265
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTCACACCGA380560.18413660443217814No Hit
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT342650.1657935871050185No Hit
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT330080.15971150512658544No Hit
ATAGATATTAATTTTATTCTTAAAATAAAAAATATTTTTCATAAACAATC297850.1441167953282643No Hit
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG259240.12543507812959287No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT245680.1188739777614503No Hit
CTGGCTCACACCGATCTAAACTCAAATCATGTAAGATTTTAATAATCGAA229420.11100646360319083No Hit
GTCTTTTCGTCCCATAAATTTATTTAAGTTTTCTTACTTAAAAAATAAAT220190.1065404638688283No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC220110.10650175531208411No Hit
ATTTTATTCTTAAAATAAAAAATATTTTTCATAAACAATCCTTTCGTACA214390.10373409350487353No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATG147900.028.284092145
GGTCATA289550.019.9816684
CGTATGG153850.018.660887145
GAGGTCA295500.018.0085812
AGGTCAT300100.017.9018693
ATGCCGG203650.017.266008145
TGCCGTG185850.017.125237145
TAATCCC338150.017.0453119
TTAAACC187150.016.9296637
TAAACCT186200.016.2371148
GTCATAA385000.015.2915295
CGAGGTC358400.014.9692271
GTCCCAT254400.014.7055029
TAGATAT338750.014.1897622
CTCATTT289150.013.9157131
CGTCCCA271450.013.7285848
GGATAGT97950.013.6191211
GATATTA387950.013.474484
GTATCTG264700.013.146855145
AAACCTT236100.013.0508919
Produced by FastQC (version 0.11.5)