FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n01_bulk_13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n01_bulk_13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23107654
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTCACACCGA438230.18964711865600895No Hit
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG393290.17019901717413632No Hit
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT376550.16295466428569513No Hit
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT371300.16068268981351375No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC354820.1535508537560758No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT348000.15059945072745162No Hit
ATAGATATTAATTTTATTCTTAAAATAAAAAATATTTTTCATAAACAATC302720.13100421185119007No Hit
GTCTTTTCGTCCCATAAATTTATTTAAGTTTTCTTACTTAAAAAATAAAT266500.11532975177835016No Hit
CTGGCTCACACCGATCTAAACTCAAATCATGTAAGATTTTAATAATCGAA263720.11412668720069982No Hit
ATTTTATTCTTAAAATAAAAAATATTTTTCATAAACAATCCTTTCGTACA259130.11214033237644981No Hit
TTTTATTCTTAAAATAAAAAATATTTTTCATAAACAATCCTTTCGTACAA238140.1030567620581475No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG164500.021.859953145
GGTCATA317850.021.6015854
GAGGTCA323850.019.700572
AGGTCAT328250.019.4369163
CGTATGG95500.019.28252145
TAATCCC373950.018.186349
TGCCGTG170000.017.655699145
TAAACCT205350.016.417888
TTAAACC224600.016.2697737
GTCATAA430850.016.0202665
CGAGGTC401500.015.312551
TCTAGAC403250.014.8333857
TAGATAT368600.014.3781192
GATATTA412200.013.7196964
CTCATTT323500.013.6035291
ATAATCC519200.013.5594898
CATTCTA508850.013.2084137
CGTCCCA303650.013.180288
CATAATC531900.013.1403747
GTCCCAT304650.013.0655359
Produced by FastQC (version 0.11.5)