FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n01_bulk_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n01_bulk_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23920478
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT413100.17269721783987763No Hit
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTCACACCGA393420.16446995749834095No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT348480.14568270751111245No Hit
ATAGATATTAATTTTATTCTTAAAATAAAAAATATTTTTTATAAACAATC344360.14396033390302654No Hit
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT342260.14308242502511864No Hit
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG333620.13947045707029768No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC318940.133333455961875No Hit
TTTTATTCTTAAAATAAAAAATATTTTTTATAAACAATCCTTTCGTACAA308050.12878087135215274No Hit
ATTTTATTCTTAAAATAAAAAATATTTTTTATAAACAATCCTTTCGTACA305020.12751417425688566No Hit
CTGGCTCACACCGATCTAAACTCAAATCATGTAAGATTTTAATAATCGAA292170.12214220802778271No Hit
GTCTTTTCGTCCCATAAATTTATTTAAGTTTTCTTACTTAAAAAATAAAT276900.11575855632985262No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGG164250.021.937445145
ATGCCGG236500.020.29373145
GGTCATA349500.020.0810684
TGCCGTG223300.019.058311145
GAGGTCA355500.018.6197722
AGGTCAT368800.018.02733
TAATCCC404600.017.2564269
GTCATAA464500.015.2655895
TAGATAT380950.014.8636762
CGAGGTC433200.014.8280761
CCAGTCC170850.014.0882845145
TCTAGAC381800.013.9765977
GATATTA435200.013.6277154
TGTAATG922700.013.40459145
GTCCCAT310350.013.2459089
ATAATCC552550.013.1871048
CATAATC560450.012.9366747
CGTCCCA320350.012.8778268
CTAGACT436400.012.5097831
CATTCTA487500.012.0764714
Produced by FastQC (version 0.11.5)