FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n01_bulk_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n01_bulk_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20174307
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT497370.24653634942702118No Hit
ATAGATATTAATTTTATTCTTAAAATAAAAAATATTTTTTATAAACAATC473470.23468959801196643No Hit
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT462370.22918755028363552No Hit
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTCACACCGA454770.2254203824696432No Hit
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG453250.22466694890684474No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT404920.2007107356897067No Hit
GTCTTTTCGTCCCATAAATTTATTTAAGTTTTCTTACTTAAAAAATAAAT359680.17828617359694188No Hit
CTGGCTCACACCGATCTAAACTCAAATCATGTAAGATTTTAATAATCGAA357330.17712132565445743No Hit
TTTTATTATAGATATTAATTTTATTCTTAAAATAAAAAATATTTTTTATA350880.1739241898123192No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC330310.16372805271576366No Hit
TATAGATATTAATTTTATTCTTAAAATAAAAAATATTTTTTATAAACAAT314060.15567325311347746No Hit
ATTTTATTCTTAAAATAAAAAATATTTTTTATAAACAATCCTTTCGTACA285730.14163063940684556No Hit
TTTTATTCTTAAAATAAAAAATATTTTTTATAAACAATCCTTTCGTACAA260450.12909984962556584No Hit
CTCACACCGATCTAAACTCAAATCATGTAAGATTTTAATAATCGAACAGA247110.12248747875205826No Hit
CCCAATTATATATATACTACAAAATTAAATTATGCTGTTATCCCTAAGGT241650.11978106608569007No Hit
CATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGTTATCCC220930.10951057699280575No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG154950.023.581526145
CTACATG152750.022.117582145
GGTCATA401800.021.7260994
GAGGTCA398450.020.5431522
AGGTCAT406000.020.1972943
CGTATGG89700.020.125185145
TAGATAT396050.019.95372
TAATCCC456650.019.2114269
TGCCGTG159200.018.762342145
GATATTA441400.018.5942784
ATTATAG350650.017.6173535
GGATAGT95950.017.378771
GTCATAA504100.017.2311825
CGAGGTC472050.016.7714581
TAAACCT167800.016.1602528
GTCCCAT337500.016.1118419
CGTCCCA343950.015.9576538
ATAATCC577650.015.2880118
AGATATT531250.015.2034753
CATAATC580700.015.17044457
Produced by FastQC (version 0.11.5)