FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n01_bulk_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n01_bulk_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24240139
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTCACACCGA562260.23195411544463504No Hit
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT480020.1980269172548887No Hit
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT448500.18502369148955786No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT406800.16782081983935818No Hit
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG399970.16500317923094418No Hit
ATAGATATTAATTTTATTCTTAAAATAAAAAATATTTTTCATAAACAATC394560.16277134384419165No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC389400.16064264317956262No Hit
ATTTTATTCTTAAAATAAAAAATATTTTTCATAAACAATCCTTTCGTACA341880.14103879519832785No Hit
GTCTTTTCGTCCCATAAATTTATTTAAGTTTTCTTACTTAAAAAATAAAT337940.13941339197766153No Hit
TTTTATTCTTAAAATAAAAAATATTTTTCATAAACAATCCTTTCGTACAA317510.13098522248572914No Hit
CTGGCTCACACCGATCTAAACTCAAATCATGTAAGATTTTAATAATCGAA301900.12454549043633785No Hit
CCCAATTATATATATACTACAAAATTAAATTATGCTGTTATCCCTAAGGT256230.10570483939881699No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTATG184400.028.622261145
GGTCATA389650.020.4124854
GAGGTCA400900.018.6813982
AGGTCAT407000.018.5086333
TAATCCC454750.017.7608559
GTCCCAT355900.016.0731289
GTCATAA512400.015.9470875
CGTCCCA363850.015.7819428
TAGATAT397250.015.6044092
GATATTA446200.015.1605874
CGAGGTC483100.015.0522961
TCGTCCC397200.015.00470357
ATAATCC602200.013.68917758
ATTATAG366600.013.587235
TCTAGAC452300.013.4012257
CATAATC621100.013.1327537
GGATAGT113350.012.8562221
CGTACAA611850.012.4781455
ATTCTAG553000.012.3111358
CTAGACT509750.012.3026231
Produced by FastQC (version 0.11.5)