FastQCFastQC Report
Fri 19 Jun 2020
H5GFLAFX2_n02_9251-3h-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFLAFX2_n02_9251-3h-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5313184
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG66130.12446397489716149No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG17000.023.67283838
TAAGGGG19300.022.30227738
AGGGGTG19700.017.94090340
AAGGGGG96850.017.5239239
GGGGGTA21600.016.6874120
GGGAGAG36100.016.3004745
ATAGGGG18600.015.61485537
GGGGTAA21500.015.30012121
GAGCGGG7700.015.0033479
ATAAGGG27700.014.90647737
GGGAAAA126700.014.85973722
CGGGGGA7650.014.63882749
GGGGGCG34250.014.40600644
GTGGGGA28450.014.39200619
AAGGGGT20350.014.27259239
AGGGGGG141650.014.13085940
AGCGGGG7700.014.09272810
GGGCGGG31850.014.06377646
AAAGGGG111250.013.99811670
GTGGGCG9500.013.99732444
Produced by FastQC (version 0.11.5)