FastQCFastQC Report
Fri 19 Jun 2020
H5GFLAFX2_n02_9251-3h-chipHop1ML.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFLAFX2_n02_9251-3h-chipHop1ML.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5232128
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG200690.3835724202465995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG11300.020.74895138
TAAGGGG12600.015.55311138
AAGGGGG59350.014.62252739
AGGGGGG83600.012.18086640
ATAGGGG13850.011.36983637
GGGAGAG25250.010.6805755
AAAGGGG68950.010.55681669
CGGGGGA5057.133167E-810.39500249
GGGAAAA99800.09.3975922
GTGGGGA20900.09.377233519
GCGGGGA8252.3646862E-119.33240148
AGGGGTG14700.09.28407840
GGGGAAA82150.09.24409521
GGGGAGA39800.09.14455322
GGGGGTA15600.08.97320620
GGGGGAA78650.08.94388450
GGGGTAA16450.08.72228321
GGGCGGG20000.08.57373446
TGGGGGA15400.08.408010520
GGCGGGA7806.6909706E-88.07573147
Produced by FastQC (version 0.11.5)