FastQCFastQC Report
Fri 19 Jun 2020
H5GFLAFX2_n02_7797-3h-notag-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFLAFX2_n02_7797-3h-notag-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2407407
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG16250.013.781445539
AGGGGTG4253.9835868E-1013.17343940
TAGGGGG3305.1139796E-712.72540338
AGGGGGG19700.012.07843940
GGGGGAA19650.011.93208920
AAAGGGG20250.011.06010338
GGGGGAG17200.010.9949825
GGGGAAA23350.010.64083521
GGGAGGG10800.09.726257
GGGGAGG11550.09.7023996
GGGAGAG8001.2732926E-119.630795
GGGGGTA4005.936686E-59.62358720
GGGAAAA30550.09.39305622
GAGGGGG13100.09.3532699
GGGGGCG5653.6648453E-79.28989845
GGAGGGG12300.09.1088958
GGGGGGC6806.360278E-88.74780644
CGGGGGG3750.00340616388.40103512
GTGGGGG11750.08.33833444
GGGCGGG4653.050408E-48.27835447
Produced by FastQC (version 0.11.5)