FastQCFastQC Report
Fri 19 Jun 2020
H5GFLAFX2_n02_7797-3h-notag-chipFlag.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFLAFX2_n02_7797-3h-notag-chipFlag.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4178943
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG116480.2787307699578577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG17850.026.07483938
TAAGGGG16400.025.39284338
AGGGGTG18000.019.44106740
ATAGGGG17100.018.21386337
AAGGGGT18450.017.44954339
TAAAAGG37700.017.35834936
GGGGTAA20650.017.2859621
CGGGGGA7100.017.2519749
AAGGGGG114700.017.02488370
ATAAGGG21000.016.33108137
GTGGGGA25200.016.10985619
GGGCGGG28800.016.03964846
GGGAAAA132000.016.01313622
GGGAGAG36400.015.8745975
GGGGGTA24100.015.68267320
GAGCGGG5150.015.6326559
GGGGGCG32300.015.38432544
GGGGGGC33750.015.03442643
TGGGGAA27850.014.95323520
AAAGGGG129200.014.8704269
Produced by FastQC (version 0.11.5)