FastQCFastQC Report
Fri 19 Jun 2020
H5GFLAFX2_n02_10705-3h-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFLAFX2_n02_10705-3h-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences904954
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24460.27028998159022444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG4300.034.9953338
TAAGGGG4250.025.52600738
AGGGGTG3350.025.07128140
ATAGGGG4100.024.75279637
GGGGTAA4800.022.6011521
GTGGGCG1905.638867E-1022.10231444
GGGGAGC1506.356586E-721.0088086
GGGGGTA5350.020.93178620
GGGAGCG1356.006254E-620.7471457
AAGGGGT4050.019.87389239
CGGGGGA1257.832037E-519.59738549
TATAGGG3600.018.46975736
GGAGCGG1602.5995154E-517.5054048
AAGGGGG24950.017.11194670
GGGCGGG6050.016.77462246
AAAGGGG26400.016.17208569
TGGGGAA5450.016.05290420
GGGAAAA26600.016.0504922
GGGGGCG6700.015.66955144
GGGTAAA7550.015.29597322
Produced by FastQC (version 0.11.5)