FastQCFastQC Report
Fri 19 Jun 2020
H5GFLAFX2_n02_10705-3h-chipFlag.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFLAFX2_n02_10705-3h-chipFlag.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1922578
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55380.2880507318818794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG16700.035.83323338
TAAGGGG13200.026.51138538
CGGGGGA4200.024.997149
GTAGGGG8550.024.14799137
TGGGGGA11700.022.73181520
ATAGGGG13000.022.61158637
GGGGAAT17700.021.55117451
TAAAGGG15250.021.34067736
AAGGGGT11150.021.02785539
AGGGGTG12450.020.79971740
TATAGGG9300.020.31916836
GGGAGCG2252.1100277E-1020.2298777
GAGCGGG2705.456968E-1219.4477569
TAAAAGG19950.019.29500636
ATAAGGG14500.018.82442337
TATAAGG10600.018.81759536
GGGGTAA15850.017.88389621
TTAGGGG6850.017.88020737
TGGGGGC3153.6379788E-1217.7747943
AAGGGGG96200.017.71624270
Produced by FastQC (version 0.11.5)