FastQCFastQC Report
Fri 19 Jun 2020
H5GFLAFX2_n02_10704-3h-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFLAFX2_n02_10704-3h-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1448836
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26670.18407880533062404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGTG4850.022.36686340
TAAGGGG4350.019.30799338
GGGGTTG5500.019.08924316
TAGGGGG4600.018.25864438
AAGGGGT5750.018.25738339
GGGGGTT5700.017.80546415
AAGGGGG25600.017.63335239
GGGTTGG5900.017.20188717
GAGGGGT3800.016.57750114
AGTGGGG5300.016.50628340
GGGAGAG9100.016.1596897
AGGAGGG5750.015.82634412
GAGAGGG10550.015.5948269
AGGGGGG36600.015.10640640
GGGAAAA37300.014.73061822
GGAGAGG9750.014.7217478
GGGGGAG32800.014.3064225
AGAGGGG11600.014.18224410
GGGGAGC2759.113064E-714.0069986
AGCGGGG2851.3691297E-613.50992510
Produced by FastQC (version 0.11.5)