FastQCFastQC Report
Fri 19 Jun 2020
H5GFLAFX2_n02_10704-3h-chipFlag.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFLAFX2_n02_10704-3h-chipFlag.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2087026
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55350.2652099207197227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGGG10050.030.2929738
TAGGGGG8000.028.432338
AGGGGTG8200.025.60507440
AAGGGGT9600.021.50648339
ATAAGGG12500.021.2761137
GAGAGGG16400.020.7043829
GGGGGTA11800.020.1682220
CGGGGGA3550.019.71706849
GGGGTAA12300.019.6329121
GAGCGGG3400.019.5618119
TAAAAGG21150.019.52455336
AAGGGGG57700.018.3155439
GTGGGCG3350.017.75794644
GGGAAAA77550.017.60043722
TATAAGG11150.017.57610336
GGGAGAG20100.017.0730135
GCGGGGA6200.016.93441848
GGAGAGG17050.016.8374338
GGGCGGG16350.016.69539546
TGGGGAA14900.016.67680220
Produced by FastQC (version 0.11.5)