FastQCFastQC Report
Fri 19 Jun 2020
H5GFLAFX2_n02_10703-3h-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFLAFX2_n02_10703-3h-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1833535
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26270.1432751488245384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG19800.018.20302639
TAAGGGG5450.017.33607138
AGGGGGG25650.015.82494540
GGGGGTA4950.015.55379720
CCAGTCG1750.001019432213.99803661
GGGAGAG7300.012.95292955
GGGGCGG6550.012.82157845
TGGGGGA4852.4374458E-1012.26665620
GAGGGGG15700.012.263449
GGGAAAA28650.012.21502422
GGGGGCG5751.8189894E-1212.17120844
GGGCGGG4902.910383E-1012.14148446
GGGAGGG13850.012.134227
GGGGAGA11000.012.0987844
AAAGGGG23150.012.09267638
TAGGGGG3953.5749144E-711.51674438
GGGGGAA23400.011.51579220
GGAGGGG14250.011.3015948
GGGGAGG14900.011.045656
GGGGAAA24650.010.50591221
Produced by FastQC (version 0.11.5)