FastQCFastQC Report
Fri 19 Jun 2020
H5GFLAFX2_n02_10703-3h-chipFlag.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFLAFX2_n02_10703-3h-chipFlag.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3428833
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG7050.017.37257438
AGGGGTG8650.016.99023440
AAGGGGG36400.016.63071639
GGGGGTA9150.016.0636820
GGGGTAA10350.014.20122621
GGGAGAG16800.013.9674895
AGGGGGG48600.013.82391940
TAAGGGG10450.013.72943938
GGGGAGA23550.013.68240454
GGGGGTG24600.012.8039315
GGGTGGG25000.012.73905817
GGGGTGG26750.012.69064916
AAAGGGG45600.012.66224370
AGGAGGG10350.012.512794512
GGGGGGT29100.012.38740114
GGGGGAA43000.012.37081750
TGGGGGG22950.012.04510645
GGGAAAA56300.011.87249222
CGGGGGA3601.46409E-611.665462549
TAAAAGG25050.011.59459536
Produced by FastQC (version 0.11.5)