FastQCFastQC Report
Fri 19 Jun 2020
H5GFLAFX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFLAFX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3250015
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2784538.567745071945822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG34050.056.6557211
CGTCTGA33550.056.56508316
ACACGTC33800.056.34493613
CACACGT34750.056.11569212
ACGTCTG33850.056.05945215
CACGTCT33800.055.83075714
GAACTCC34850.054.1879521
AGCACAC36500.053.2344110
TCCAGTC32750.052.7661925
CAGTCAC28750.052.1647627
GTCTGAA37250.051.31966417
CCAGTCA32400.051.24467526
GAGCACA38800.050.991149
AGAGCAC39950.049.959258
CTCCAGT36550.049.86070324
TGAACTC38300.049.7509820
CTGAACT37800.049.65515519
TCTGAAC38400.048.97492218
TCGGAAG42350.048.3565373
GATCGGA41800.048.168491
Produced by FastQC (version 0.11.5)