FastQCFastQC Report
Fri 19 Jun 2020
H5GFLAFX2_n01_MMS-control-pool.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFLAFX2_n01_MMS-control-pool.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20599744
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGCT43750.027.59960231
GTCACGG45200.026.79164929
TCACGGC47200.025.6564130
AGTCACG48500.025.25736828
CGGCTAC50950.023.76805733
GCACACG52850.023.57581911
ACGGCTA56350.021.98726332
CATCTCG52550.021.44624539
GCCGTCT56300.021.3229649
CACACGT59050.021.04118712
ACGTCTG60150.020.88914115
TGCCGTC59250.020.61573648
GATCGGA62400.020.0859811
ACACGTC63850.019.51420613
CACGTCT64700.019.25783714
GTATGCC65900.018.74810645
CGTCTGA67600.018.2245916
GGCTACA67850.018.20902334
AGCACAC70200.017.9484610
CAGTCAC72050.017.19614427
Produced by FastQC (version 0.11.5)