FastQCFastQC Report
Fri 19 Jun 2020
H5GFLAFX2_n01_9251-3h-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFLAFX2_n01_9251-3h-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5313184
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT1248732.3502479868944874TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG147550.066.86783635
GTCCCGA146950.066.54542536
CCCGTCC152200.065.58375533
ACCCGTC152450.065.4532432
CCGTCCC152200.065.2618234
TCACCCG153750.065.05916630
CACCCGT154250.064.75751531
CCCGATC141850.064.5960238
TCCCGAT148200.064.0245937
GTCACCC156650.063.9217829
GCCGTCT154100.063.70765351
GATCTCG144950.063.21452741
CCGATCT144900.063.06725739
CGATCTC144900.063.04310240
TGCCGTC157700.063.0079150
CGTATGC159000.062.58080346
CAGTCAC160350.062.5559527
GTATGCC159350.062.44334847
CCAGTCA160650.062.4391326
ACGTCTG162150.062.22846615
Produced by FastQC (version 0.11.5)