FastQCFastQC Report
Fri 19 Jun 2020
H5GFLAFX2_n01_9251-3h-chipHop1ML.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFLAFX2_n01_9251-3h-chipHop1ML.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5232128
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC399210.7629973884430962TruSeq Adapter, Index 7 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG62550.1195498275271553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC56450.057.47514348
ACACGTC73200.057.47217613
ACGTCTG73600.057.1598315
GCCGTCT50550.057.12116249
GCACACG73350.056.9729211
CGTCTGA74850.056.20525416
CATCTCG58800.056.07094239
CACACGT74600.056.01827612
GTATGCC62300.055.8422145
TATGCCG61900.055.80726646
GAACTCC74950.055.66329221
CACGTCT75550.055.59183514
CCAGTCA74950.055.52319726
CGTATGC62000.055.49145544
CAGTCAC75500.055.3505127
TCCAGTC75250.055.25533325
AGTCACC77500.053.92210828
AGCACAC78800.053.25461210
CCAGATC78650.053.04467433
CTCGTAT59250.052.80966642
Produced by FastQC (version 0.11.5)