FastQCFastQC Report
Fri 19 Jun 2020
H5GFLAFX2_n01_7797-3h-notag-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFLAFX2_n01_7797-3h-notag-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2407407
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC140920.5853600990609399TruSeq Adapter, Index 12 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29780.1237015593956485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC19800.053.2065245
TATGCCG20500.051.21898346
GCCGTCT20000.050.57447449
TGCCGTC20700.050.3859548
ACGTCTG22000.050.11311315
ACACGTC22450.049.5763213
CACGTCT22850.048.40212614
ATGCCGT21500.048.34833547
CGTATGC21900.048.26433644
CGTCTGA22900.047.99076516
CACACGT23300.047.6175312
CAGTCAC23200.047.52105727
CCAGTCA23200.047.52105726
TCCAGTC23550.046.9634225
GTCACCT23550.046.81480429
GCACACG23950.046.32519511
AGTCACC24150.045.79662728
ATCTCGT21050.045.22518540
GAACTCC24100.045.16550421
CTCGTAT21600.044.7217642
Produced by FastQC (version 0.11.5)