FastQCFastQC Report
Fri 19 Jun 2020
H5GFLAFX2_n01_7797-3h-notag-chipFlag.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFLAFX2_n01_7797-3h-notag-chipFlag.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4178943
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC1205092.8837196391527717TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGC88300.21129745009683068TruSeq Adapter, Index 2 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG87250.20878485301187405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC141900.064.523248
GCCGTCT131000.064.4683549
CCGATGT167800.063.99174533
CAGTCAC168750.063.9840827
GTATGCC149150.063.94458845
GTCACCG169150.063.70862629
ACGTCTG171550.063.63340815
ACACGTC171600.063.6148713
TATCTCG148650.063.54750439
TATGCCG148550.063.4960446
ATGCCGT144000.063.46070547
CACCGAT170050.063.4126131
CGTCTGA172150.063.37096816
CGATGTA169700.063.35777734
CACGTCT172400.063.33997714
ACCGATG170200.063.27446432
TCCAGTC170300.063.25786625
TCACCGA170550.063.20617730
CTCGTAT143800.063.0621942
CCAGTCA171300.062.97030626
Produced by FastQC (version 0.11.5)