FastQCFastQC Report
Fri 19 Jun 2020
H5GFLAFX2_n01_10705-3h-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFLAFX2_n01_10705-3h-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences904954
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT125761.3896838955350217TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGTAT22580.24951544498394396TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTTT19590.21647509155161476TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGGAT17550.19393250927671463TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGGTT12580.13901259069521765TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGGAT11750.12984085378925336TruSeq Adapter, Index 15 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10360.11448095704312043No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGGTT9680.10696676295148703TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAGTC35300.063.25758725
ACGTCTG35750.062.95084815
CAGTCAC35600.062.92115427
CACGTCT36200.062.55504614
GCACACG36400.062.4036511
CCAGTCA35900.062.2003626
CACATGT36250.062.1791131
ACACGTC36650.061.97797813
GTCTGAA36450.061.9339617
TCACATG36400.061.92288230
CGTCTGA36350.061.91177416
CACACGT36800.061.82045412
ATGTCAG36400.061.6344234
AGTCACA36700.061.51206628
ACTCCAG36400.061.44211223
TGTCAGA36750.061.237935
GTCACAT36850.061.16670229
ACATGTC36850.061.16670232
CTCCAGT36600.061.1063624
AGCACAC37300.061.08559410
Produced by FastQC (version 0.11.5)