FastQCFastQC Report
Fri 19 Jun 2020
H5GFLAFX2_n01_10705-3h-chipFlag.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFLAFX2_n01_10705-3h-chipFlag.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1922578
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1296746.744797870359485TruSeq Adapter, Index 6 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30770.16004552221028223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCC140950.067.83842530
GTCACGC141550.067.6992329
TATCTCG138700.067.5762739
AGTCACG142650.067.5206728
GTATGCC140800.067.3886945
CACGCCA142200.067.3651631
GCCGTCT138000.067.20891649
TGCCGTC140450.067.13299648
ATGCCGT140800.067.0158247
CAGTCAC143350.066.94681527
CCAGTCA143300.066.799226
CGTATGC142350.066.75326544
TATGCCG142000.066.67132646
CTCGTAT140800.066.5435342
TCGTATG142450.066.2150143
TCCAGTC144600.066.1744525
CGCCAAT144350.066.1678233
TCTCGTA141600.066.1675841
ATCTCGT141950.066.1030640
ACGCCAA145000.066.06431632
Produced by FastQC (version 0.11.5)