FastQCFastQC Report
Fri 19 Jun 2020
H5GFLAFX2_n01_10704-3h-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFLAFX2_n01_10704-3h-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1448836
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT307002.1189423785714876TruSeq Adapter, Index 14 (97% over 44bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19260.1329343003624979No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTCCG39950.062.903234
TGCCGTC35700.062.74466350
GTTCCGT40050.062.39657635
GCCGTCT33950.062.16451651
CCGTATC39500.062.0248938
CAGTCAC41100.061.8243927
TATGCCG37050.061.7809548
TATCTCG36600.061.77552841
TCCAGTC41300.061.4402525
GAACTCC41700.061.27055721
AGTCACA41700.061.18662628
GTATGCC37800.060.92550747
CCAGTCA41650.060.8399226
CGTATCT38050.060.7091739
ATGCCGT37150.060.67252749
TCCGTAT40800.060.47752837
GCACACG42400.060.25901811
CGTCTGA42300.060.1532516
GTCACAG42500.060.11723329
CTCGTAT37150.060.01304644
Produced by FastQC (version 0.11.5)