FastQCFastQC Report
Fri 19 Jun 2020
H5GFLAFX2_n01_10704-3h-chipFlag.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFLAFX2_n01_10704-3h-chipFlag.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2087026
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC471752.260393497733138TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC170180.8154186866862223TruSeq Adapter, Index 5 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47760.2288423814557174No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCGCGTATGC27380.13119146575078605TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGTATGC26440.1266874490303427TruSeq Adapter, Index 5 (97% over 41bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACAC88100.065.15182529
GCCGTCT72350.064.967549
GTATGCC80700.064.8390645
TATGCCG80300.064.4631146
CACACAG89450.064.3250531
CACAGTG89400.064.2435833
CGTATGC81150.064.0482144
ATGCCGT78500.063.8903447
TCCAGTC89550.063.82330325
TGCCGTC78500.063.71199848
CCAGTCA90450.063.3430326
CAGTCAC90650.063.28048327
GATCTCG55800.063.2259639
AGTCACA91050.063.19468328
ACACAGT91200.063.09074432
CGTCTGA92500.062.6959516
TCACACA91800.062.67838730
GAACTCC91800.062.4877621
AGTGATC60950.062.47609336
CCGTCTT76900.062.30685450
Produced by FastQC (version 0.11.5)