FastQCFastQC Report
Fri 19 Jun 2020
H5GFLAFX2_n01_10703-3h-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFLAFX2_n01_10703-3h-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1833535
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT187061.0202150490718749TruSeq Adapter, Index 13 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20380.11115140970856842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT26700.057.02182812
GCACACG26800.056.67846311
CACGTCT27600.055.5428514
ACGTCTG27600.055.41604215
CACAGTC27300.054.74297331
CGTCTGA28100.054.679116
ACACGTC28200.054.485213
TATGCCG27400.054.1599748
TGCCGTC27150.054.01412650
GAACTCC27800.054.0101821
CTCGTAT26250.053.9993944
GCCGTCT27000.053.9253251
AGCACAC28500.053.78886810
GTATGCC27500.053.70848547
TCCAGTC27650.053.6702825
CGTATGC27950.053.0942146
ATCTCGT26750.052.9900542
GTCTGAA29050.052.6500117
GAGCACA29150.052.230689
CAGTCAC28500.052.19239427
Produced by FastQC (version 0.11.5)