FastQCFastQC Report
Fri 19 Jun 2020
H5GFLAFX2_n01_10703-3h-chipFlag.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFLAFX2_n01_10703-3h-chipFlag.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3428833
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC384021.1199728887350304TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGACC48600.059.91689732
GCCGTCT48200.059.9058449
TGCCGTC48650.059.4956148
GTATGCC49500.058.89820545
ATGCCGT50650.057.56093247
CAGTCAC51750.057.14899427
TATGCCG51650.056.44648746
CCAGTCA52600.056.29202726
TCCAGTC52800.056.14508425
CGTATGC52050.056.01270344
AGTCACT53050.055.74854728
CACTGAC52950.055.5894431
CTCGTAT50750.055.58546442
CTGACCA52900.054.98036633
CGTCTGA53850.054.8553516
ATCTCGT50250.054.67588840
TCGTATG53250.054.48753443
TCTCGTA50950.054.4742541
ACGTCTG54600.054.10184515
GTCACTG54750.054.01754429
Produced by FastQC (version 0.11.5)