FastQCFastQC Report
Wed 29 Jan 2020
H5GFKBGXF_n02_Salt_NSF_Plate_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFKBGXF_n02_Salt_NSF_Plate_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326601049
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63328981.9390317389948126No Hit
GTGTTATACCGGTGGCTTCAGGGGGTATTCATGTTTGGCATATGCCAGCT6897860.2112014037040034No Hit
TCCTTGGGGTAATGCACCTGGTGCAGCAGCTAATCGGGTGGCTTTAGAAG4331930.13263674483788937No Hit
GTCATATATAGCCTCGTTTAATTGTTCATCTCTGATTCGATGATGTCTCC4246560.13002285243731718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA805950.020.9929941
GTATCAA2640400.020.9463881
GTTATAC2296450.018.8578833
GGACCAT1629550.018.7087146
ATACCGG2375700.018.2851526
TACCGGT2431700.017.782647
TCAGGGG2327250.017.69800418
GGGGGTA2333950.017.65954221
GGGGTAT2321200.017.29877322
CAGGGGG2401400.017.16071719
GGGTATT2364200.016.9888323
GGTATTC2355700.016.98762924
TGGACCA1952150.016.8398155
GGCTTCA2457350.016.77355214
AGGGGGT2458900.016.7110920
TGTTATA2675450.016.6606862
TATACCG2739500.016.578125
GTCATAT1882600.016.2326031
GTACTGG1457700.016.1541731
TGGCATA2348450.016.11938936
Produced by FastQC (version 0.11.5)