FastQCFastQC Report
Wed 29 Jan 2020
H5GFKBGXF_n01_Salt_NSF_Plate_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFKBGXF_n01_Salt_NSF_Plate_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326601049
Sequences flagged as poor quality0
Sequence length20
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG88465082.7086587832729223No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCTCT2863000.013.878754
CCACACT3165400.013.8423182
TTCCACC2658600.013.8359592
TCAGAAC3229400.013.8265862
AACTACC3239400.013.8247336
CCTCAGT3183250.013.8154856
TCAGAGC4347050.013.8107551
CCTCTGA2848450.013.8093244
GTCCTCT2855350.013.798232
TCCACAC3153000.013.7924531
TCGACCA2572200.013.791262
TCCTTAC2717200.013.7903354
AATCCTT2716300.013.7897052
GGTCCAA3998450.013.7854853
ACCTCTC2836000.013.7847823
TCGACTT2708300.013.7826126
CGCATCC2485800.013.7810955
CGCATCT2652900.013.777465
CTTCGTC3640200.013.7715432
AGGACAC3283600.013.7713665
Produced by FastQC (version 0.11.5)