FastQCFastQC Report
Wed 6 Oct 2021
H5G5CBGXK_n01_JK47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5G5CBGXK_n01_JK47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26048662
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGGTTGG698160.2680214438653317TruSeq Adapter, Index 7 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGGGTGG589870.2264492510210313TruSeq Adapter, Index 7 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGGTTTG385470.14798072929811137TruSeq Adapter, Index 7 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC372690.1430745272060423TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGTTTGG299680.11504621619336916TruSeq Adapter, Index 7 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGGGTTG272170.10448521309846931TruSeq Adapter, Index 7 (97% over 42bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)